After a shaky display in the opener, keeper Chalkias' place in the starting XI could come under threat from Sifakis.
Avraam Papadopoulos' tournament is sadly already over after he returned to Greece having torn the anterior cruciate ligament in his knee against Poland.
His starting defensive partner Sokratis is suspended after a somewhat harsh dismissal in the first half of Friday's match, meaning youngster Kyriakos Papadopoulos may be partnered by veteran midfielder Katsouranis in defence.
Salpingidis will surely start after an excellent goalscoring cameo against the co-hosts, while Fourtounis will also hope to feature.
The Czech's have no fresh injury concerns going into Tuesday's game but will have been hurt by a crushing 4-1 defeat to Russia on the opening day.
Goalscorer Vaclav Pilar was impressive despite the defeat and will keep his place on the left wing.
Elsewhere the likes of Petrzela and Hubschmann will hope to start in place of Rezek and Jiracek, while Pekhart, Lafata and Necid are waiting in the wings if Milan Baros is dropped.
The Czech Republic will feel that they may need to win this game to have any chance of qualifying, with a draw perhaps not good enough to take into their final game with Poland having conceded 4 to Russia last time out.
Greece will feel relieved after they looked dead and buried at half time against Poland, trailing 1-0 and with a man down. The draw may have felt like a victory had Karagounis not missed a penalty that could have won them the game.
Santos' side face group favourites Russia last so are also likely to look to seal all 3 points knowing that Dick Advocaat's team are in excellent form, which should mean that there are goals in this match.
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