Maicon and James Milner both missed the game with Arsenal due to minor foot injuries but could be in contention to come into a much changed line-up with Mancini likely to rotate his entire starting XI.
Gareth Barry could build up his fitness against his former club, while new signings Nastasic, Rodwell and Sinclair could all feature.
Mario Balotelli is likely to start having scored 5 goals in 3 appearances against Villa in the past, while a number of youngsters could also be handed a chance.
Paul Lambert is renowned for making changes to his side after a defeat and the second half performance against Southampton will have given him cause for concern, with Stephen Ireland suffering a suspected broken arm.
Among the likely changes will be left-back Joe Bennett, while Nathan Baker could be given another chance to impress alongside Ron Vlaar and Herd and Delph will also hope to feature.
The likes of Holman and El Ahmadi may be rested, with Agbonlahor and N'Zogbia vying for a start, while new signings Westwood and Bowery are ineligible having featured in the earlier rounds of the cup with their former teams
With both sides likely to make changes from their weekend line-ups this will be a chance for a few players to stake a claim for a regular place, though City will still be strong favourites even with a weakened XI.
Villa will fear the inclusion of Mario Balotelli, who tends to save his best performances against them, and they will need to improve massively at the back having conceded 16 goals in their last 5 away matches at City.
Villa have led 1-0 at half time in each of their last 4 matches but expect City to be dominant early on and make plenty of chances. The game could become fragmented in the second half with a raft of changes likely, particularly from the hosts.
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